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RNA disruption is a widespread phenomenon associated with stress-induced cell death in tumour cells 
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Effect of doxorubicin on cell viability/death and RNA integrity in three non-tumourigenic cell lines. Human umbilical vein endothelial cells (HUVECs) (left panels), mouse <t>NIH3T3</t> fibroblast cells (middle panels) and human MCF-10A breast epithelial cells (right panels) were treated with doxorubicin for 72 h. ( a ) RNA disruption assay. Total RNA was isolated from cells, and RNA disruption was quantified using the RDA. ( b ) Cell counting assay. Total cells were counted using a haemocytometer following drug treatment. Untreated cells were counted prior to treatment (‘0 h’) to provide a baseline count. ( c ) DNA content analysis. Drug-treated cells were collected, washed, fixed and stained with propidium iodide then analysed by flow cytometry. Data are presented as means ± standard deviation, with individual data points shown in red. Groups labelled with an asterisk were significantly greater (panels a and c) or significantly lower (panel b) than the ‘0 h’ (panel b) or untreated (panels a and c) control. See Supplementary Table for detailed results of each statistical test.
RNA disruption is a widespread phenomenon associated with stress-induced cell death in tumour cells Scientific Reports, 2023 Jan 31
"Effect of doxorubicin on cell viability/death and RNA integrity in three non-tumourigenic cell lines. Human umbilical vein endothelial cells (HUVECs) (left panels), mouse <t>NIH3T3</t> fibroblast cells (middle panels) and human MCF-10A breast epithelial cells (right panels) were treated with doxorubicin for 72 h. ( a ) RNA disruption assay. Total RNA was isolated from cells, and RNA disruption was quantified using the RDA. ( b ) Cell counting assay. Total cells were counted using a haemocytometer following drug treatment. Untreated cells were counted prior to treatment (‘0 h’) to provide a baseline count. ( c ) DNA content analysis. Drug-treated cells were collected, washed, fixed and stained with propidium iodide then analysed by flow cytometry. Data are presented as means ± standard deviation, with individual data points shown in red. Groups labelled with an asterisk were significantly greater (panels a and c) or significantly lower (panel b) than the ‘0 h’ (panel b) or untreated (panels a and c) control. See Supplementary Table for detailed results of each statistical test. "
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